Structural basis of nucleosome transcription mediated by Chd1 and FACT
2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Farnung, Lucas; Ochmann, Moritz; Engeholm, Maik; Cramer, Patrick
- Abstract
- Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is facilitated by the chromatin remodeler Chd1 and the histone chaperone FACT when the elongation factors Spt4/5 and TFIIS are present. We report cryo-EM structures of transcribing Saccharomyces cerevisiae Pol II-Spt4/5-nucleosome complexes with bound Chd1 or FACT. In the first structure, Pol II transcription exposes the proximal histone H2A-H2B dimer that is bound by Spt5. Pol II has also released the inhibitory DNA-binding region of Chd1 that is poised to pump DNA toward Pol II. In the second structure, Pol II has generated a partially unraveled nucleosome that binds FACT, which excludes Chd1 and Spt5. These results suggest that Pol II progression through a nucleosome activates Chd1, enables FACT binding and eventually triggers transfer of FACT together with histones to upstream DNA.
- Issue Date
- 2021
- Journal
- Nature Structural & Molecular Biology
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Cramer
- ISSN
- 1545-9993
- eISSN
- 1545-9985
- Language
- English