Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture
2020 | book part
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Dual-Color Metal-Induced Energy Transfer (MIET) Imaging for Three-Dimensional Reconstruction of Nuclear Envelope Architecture
Chizhik, A. I.; Chizhik, A. M.; Ruhlandt, D.; Pfaff, J.; Karedla, N.; Gregor, I.& Kehlenbach, R. H. et al. (2020)
In:Hancock, Ronald (Ed.), The Nucleus pp. 33-45. (3. ed.). New York: Springer. DOI: https://doi.org/10.1007/978-1-0716-0763-3_4
Documents & Media
Details
- Authors
- Chizhik, Alexey I.; Chizhik, Anna M.; Ruhlandt, Daja; Pfaff, Janine; Karedla, Narain; Gregor, Ingo; Kehlenbach, Ralph H. ; Enderlein, Jörg
- Editors
- Hancock, Ronald
- Abstract
- The nuclear envelope, comprising the inner and the outer nuclear membrane, separates the nucleus from the cytoplasm and plays a key role in cellular functions. Nuclear pore complexes (NPCs) are embedded in the nuclear envelope and control transport of macromolecules between the two compartments. Recently, it has been shown that the axial distance between the inner nuclear membrane and the cytoplasmic side of the NPC can be measured using dual-color metal-induced energy transfer (MIET). This chapter focuses on experimental aspects of this method and discusses the details of data analysis.
- Issue Date
- 2020
- Publisher
- Springer
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P07: Dynamik von Proteinen der inneren Kernmembran - Working Group
- RG Enderlein
RG Kehlenbach (Nuclear Transport) - ISBN
- 978-1-0716-0762-6
978-1-0716-0765-7 - eISBN
- 978-1-0716-0763-3
- Language
- English
