Photonic-chip assisted correlative light and electron microscopy
2020 | journal article; research paper
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Cite this publication
Photonic-chip assisted correlative light and electron microscopy
Tinguely, J.-C.; Steyer, A. M.; Øie, C. I.; Helle, Ø. I.; Dullo, F. T.; Olsen, R. & McCourt, P. et al. (2020)
Communications Biology, 3(1) pp. 739. DOI: https://doi.org/10.1038/s42003-020-01473-4
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Details
- Authors
- Tinguely, Jean-Claude; Steyer, Anna Maria; Øie, Cristina Ionica; Helle, Øystein Ivar; Dullo, Firehun Tsige; Olsen, Randi; McCourt, Peter; Schwab, Yannick; Ahluwalia, Balpreet Singh
- Abstract
- Correlative light and electron microscopy (CLEM) unifies the versatility of light microscopy (LM) with the high resolution of electron microscopy (EM), allowing one to zoom into the complex organization of cells. Here, we introduce photonic chip assisted CLEM, enabling multi-modal total internal reflection fluorescence (TIRF) microscopy over large field of view and high precision localization of the target area of interest within EM. The photonic chips are used as a substrate to hold, to illuminate and to provide landmarking of the sample through specially designed grid-like numbering systems. Using this approach, we demonstrate its applicability for tracking the area of interest, imaging the three-dimensional (3D) structural organization of nano-sized morphological features on liver sinusoidal endothelial cells such as fenestrations (trans-cytoplasmic nanopores), and correlating specific endo-lysosomal compartments with its cargo protein upon endocytosis.
- Issue Date
- 2020
- Journal
- Communications Biology
- Project
- FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala
FOR 2848 | P08: Strukturelle und funktionale Veränderungen der inneren mitochondrialen Membran axonaler Mitochondrien in vivo in einem dymyelinisierenden Mausmodell - Working Group
- RG Möbius
- ISSN
- 2399-3642
- Language
- English