SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy
2015 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy
Lukinavicius, G.; Blaukopf, C.; Pershagen, E.; Schena, A.; Reymond, L.; Derivery, E. & Gonzalez-Gaitan, M. et al. (2015)
Nature Communications, 6 art. 8497. DOI: https://doi.org/10.1038/ncomms9497
Documents & Media
Details
- Authors
- Lukinavicius, Grazvydas; Blaukopf, Claudia; Pershagen, Elias; Schena, Alberto; Reymond, Luc; Derivery, Emmanuel; Gonzalez-Gaitan, Marcos; D'Este, E. ; Hell, Stefan ; Gerlich, Daniel Wolfram; Johnsson, Kai
- Abstract
- Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
- Issue Date
- 2015
- Publisher
- Nature Publishing Group
- Journal
- Nature Communications
- ISSN
- 2041-1723
