Long-term in vivo and in vitro AAV-2-mediated RNA interference in rat retinal ganglion cells and cultured primary neurons
2005 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Long-term in vivo and in vitro AAV-2-mediated RNA interference in rat retinal ganglion cells and cultured primary neurons
Michel, U. ; Malik, I. ; Ebert, S.; Bähr, M. & Kugler, S. (2005)
Biochemical and Biophysical Research Communications, 326(2) pp. 307-312. DOI: https://doi.org/10.1016/j.bbrc.2004.11.029
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- Authors
- Michel, Uwe ; Malik, I. ; Ebert, Sandra; Bähr, Mathias ; Kugler, S.
- Abstract
- Viral vector-based expression of small interfering RNAs is a promising tool for gene regulation, both in cultured cells and in animal models. In this study, we analysed the ability of adeno-associated virus-2 to function as an RNAi vector in cultured primary hippocampal neurons in vitro and in retinal ganglion cells in vivo. We demonstrate a long-lasting, highly efficient, and specific down-regulation of gene expression in vivo and in vitro by the use of bicistronic vectors. This is the first evidence of a cell type-specific long-term (more than three-month-long) RNAi in the eye. Furthermore, our results constitute the prerequisite for the use of this technique in models of neurodegeneration and neuroregeneration in vivo and in vitro. (C) 2004 Elsevier Inc. All rights reserved.
- Issue Date
- 2005
- Publisher
- Academic Press Inc Elsevier Science
- Journal
- Biochemical and Biophysical Research Communications
- ISSN
- 0006-291X