Adenovirus-mediated expression of ciliary neurotrophic factor (CNTF) rescues axotomized rat retinal ganglion cells but does not support axonal regeneration in vivo
2000 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Cite this publication
Adenovirus-mediated expression of ciliary neurotrophic factor (CNTF) rescues axotomized rat retinal ganglion cells but does not support axonal regeneration in vivo
Weise, J.; Isenmann, S.; Klocker, N.; Kugler, S. ; Hirsch, S.; Gravel, C. & Bähr, M. (2000)
Neurobiology of Disease, 7(3) pp. 212-223. DOI: https://doi.org/10.1006/nbdi.2000.0285
Documents & Media
Details
- Authors
- Weise, Jens; Isenmann, Stefan; Klocker, N.; Kugler, S. ; Hirsch, S.; Gravel, C.; Bähr, Mathias
- Abstract
- Rat optic nerve (ON) transection leads to mainly apoptotic cell death of about 85% of the retinal ganglion cell (RGC) population within 14 days after lesion. in the present study, we tested the effect of adenovirally delivered CNTF (Ad-CNTF) on survival and regeneration of axotomized adult RGCs in vivo. Single intravitreal Ad-CNTF injection led to stable CNTF mRNA and protein expression for at least 18 days and significantly enhanced RGC survival by 155% when compared to control animals 14 days after ON transection. ON stump application of Ad-CNTF also resulted in an increased number of surviving RGCs. Ad-CNTF injection led to better preservation of intraretinal RGC axons but did not support regeneration of axotomized RGCs into a peripheral nerve graft. Thus, adenovirus-mediated neurotrophic factor supply is a suitable approach for reducing axotomy-induced RGC death in vivo and may constitute a relevant strategy for clinical treatment of traumatic brain injury. (C) 2000 Academic Press.
- Issue Date
- 2000
- Publisher
- Academic Press Inc
- Journal
- Neurobiology of Disease
- ISSN
- 0969-9961