Systematic Heterogeneity of Fractional Vesicle Pool Sizes and Release Rates of Hippocampal Synapses
2011 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Systematic Heterogeneity of Fractional Vesicle Pool Sizes and Release Rates of Hippocampal Synapses
Welzel, O.; Henkel, A. W.; Stroebel, A. M.; Jung, J.; Tischbirek, C. H.; Ebert, K. & Kornhuber, J. et al. (2011)
Biophysical Journal, 100(3) pp. 593-601. DOI: https://doi.org/10.1016/j.bpj.2010.12.3706
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Details
- Authors
- Welzel, Oliver; Henkel, Andreas Wolfram; Stroebel, Armin M.; Jung, Jasmin; Tischbirek, Carsten H.; Ebert, Katrin; Kornhuber, Johannes ; Rizzoli, S. O. ; Groemer, Teja Wolfgang
- Abstract
- Hippocampal neurons in tissue culture develop functional synapses that exhibit considerable variation in synaptic vesicle content (20-350 vesicles). We examined absolute and fractional parameters of synaptic vesicle exocytosis of individual synapses. Their correlation to vesicle content was determined by activity-dependent discharge of FM-styryl dyes. At high frequency stimulation (30 Hz), synapses with large recycling pools released higher amounts of dye, but showed a lower fractional release compared to synapses that contained fewer vesicles. This effect gradually vanished at lower frequencies when stimulation was triggered at 20 Hz and 10 Hz, respectively. Live-cell antibody staining with anti-synaptotagmin-1-cypHer 5, and overexpression of synaptopHluorin as well as photoconversion of FM 1-43 followed by electron microscopy, consolidated the findings obtained with FM-styryl dyes. We found that the readily releasable pool grew with a power function with a coefficient of 2/3, possibly indicating a synaptic volume/surface dependency. This observation could be explained by assigning the rate-limiting factor for vesicle exocytosis at high frequency stimulation to the available active zone surface that is proportionally smaller in synapses with larger volumes.
- Issue Date
- 2011
- Journal
- Biophysical Journal
- ISSN
- 0006-3495
- Language
- English