FORMATION OF FUNCTIONAL SYNAPSES BY REGENERATING ADULT-RAT RETINAL GANGLION-CELL AXONS IN MIDBRAIN TARGET REGIONS INVITRO
1993 | journal article; research paper. A publication with affiliation to the University of Göttingen.
Jump to: Cite & Linked | Documents & Media | Details | Version history
Documents & Media
Details
- Authors
- Bähr, Mathias ; Eschweiler, G. W.
- Abstract
- The ability of adult rat retinal ganglion cell (RGC) axons to reinnervate normal target regions was examined in vitro. In co-culture experiments, adult rat retinal explants were placed adjacent to fetal rat midbrain sections that contained the superior colliculus (SC) which is the main target for RGC axons. Adult rat RGCs regrew axons over more than 500 mum on a polylysine-laminin substrate to reach the co-cultured explants. By using neurofilament immunohistochemistry and the fluorescent dye Dil for anterograde and retrograde tracing, it was shown that (1) adult rat RGCs with a stereotyped morphology survived in explant cultures for more than 4 weeks in the presence of fetal midbrain explants, (2) regenerating RGC axons preferentially terminated within midbrain target regions, and (3) RGCs formed functional synapses. In addition, the maturation of the SC region in midbrain explants was examined histologically and ultrastructurally to demonstrate appropriate target development.
- Issue Date
- 1993
- Publisher
- John Wiley & Sons Inc
- Journal
- Journal of Neurobiology
- ISSN
- 0022-3034