The Hedgehog Receptor Patched1 in T Cells Is Dispensable for Adaptive Immunity in Mice

2013 | journal article. A publication with affiliation to the University of Göttingen.

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​The Hedgehog Receptor Patched1 in T Cells Is Dispensable for Adaptive Immunity in Mice​
Michel, K. D.; Uhmann, A.; Dressel, R.; van den Brandt, J.; Hahn, H. & Reichardt, H. M.​ (2013) 
PLoS ONE8(4) art. e61034​.​ DOI: https://doi.org/10.1371/journal.pone.0061034 

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Authors
Michel, Kai D.; Uhmann, Anja; Dressel, Ralf; van den Brandt, Jens; Hahn, Heidi; Reichardt, Holger Michael
Abstract
Hedgehog (Hh) signaling modulates T cell development and function but its exact role remains a matter of debate. To further address this issue we made use of conditional knock-out mice in which the Hh receptor Patched1 (Ptch) is inactivated in the T cell lineage. Thymocyte development was moderately compromised by the deletion of Ptch as characterized by reduced numbers of CD4 and CD8 single-positive cells. In contrast, peripheral T cells were not affected. Proliferation and IFN gamma secretion by Ptch-deficient T cells were indistinguishable from controls irrespectively of whether we used strong or suboptimal conditions for stimulation. Analysis of CTL and T-reg cell functions did not reveal any differences between both genotypes, and T cell apoptosis induced by glucocorticoids or gamma-irradiation was also similar. Surprisingly, absence of Ptch did not lead to an activation of canonic Hh signaling in peripheral T cells as indicated by unaltered expression levels of Gli1 and Gli2. To test whether we could uncover any role of Ptch in T cells in vivo we subjected the mutant mice to three different disease models, namely allogeneic bone marrow transplantation mimicking graft-versus-host disease, allergic airway inflammation as a model of asthma and growth of adoptively transferred melanoma cells as a means to test tumor surveillance by the immune system. Nonetheless, we were neither able to demonstrate any difference in the disease courses nor in any pathogenic parameter in these three models of adaptive immunity. We therefore conclude that the Hh receptor Ptch is dispensable for T cell function in vitro as well as in vivo.
Issue Date
2013
Status
published
Publisher
Public Library Science
Journal
PLoS ONE 
ISSN
1932-6203

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