Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1
2014 | journal article. A publication with affiliation to the University of Göttingen.
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Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1
Hackmann, A.; Wu, H.; Schneider, U.-M.; Meyer, K.; Jung, K. & Krebber, H. (2014)
Nature Communications, 5 art. 3123. DOI: https://doi.org/10.1038/ncomms4123
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Details
- Authors
- Hackmann, Alexandra; Wu, Haijia; Schneider, Ulla-Maria; Meyer, Katja; Jung, Klaus; Krebber, Heike
- Abstract
- Eukaryotic cells have to prevent the export of unspliced pre-mRNAs until intron removal is completed to avoid the expression of aberrant and potentially harmful proteins. Only mature mRNAs associate with the export receptor Mex67/TAP and enter the cytoplasm. Here we show that the two shuttling serine/arginine (SR)-proteins Gbp2 and Hrb1 are key surveillance factors for the selective export of spliced mRNAs in yeast. Their absence leads to the significant leakage of unspliced pre-mRNAs into the cytoplasm. They bind to pre-mRNAs and the spliceosome during splicing, where they are necessary for the surveillance of splicing and the stable binding of the TRAMP complex to spliceosome-bound transcripts. Faulty transcripts are marked for their degradation at the nuclear exosome. On correct mRNAs the SR proteins recruit Mex67 upon completion of splicing to allow a quality controlled nuclear export. Altogether, these data identify a role for shuttling SR proteins in mRNA surveillance and nuclear mRNA quality control.
- Issue Date
- 2014
- Status
- published
- Publisher
- Nature Publishing Group
- Journal
- Nature Communications
- ISSN
- 2041-1723
- Sponsor
- Deutsche Forschungsgemeinschaft; [SFB 860]