CCL5 deficiency reduces neointima formation following arterial injury and thrombosis in apolipoprotein E-deficient mice

2016 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​CCL5 deficiency reduces neointima formation following arterial injury and thrombosis in apolipoprotein E-deficient mice​
Czepluch, F. S. ; Meier, J.; Binder, C. ; Hasenfuß, G.   & Schaefer, K. ​ (2016) 
Thrombosis Research144 pp. 136​-143​.​ DOI: https://doi.org/10.1016/j.thromres.2016.06.013 

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Authors
Czepluch, Frauke S. ; Meier, Julia; Binder, Claudia ; Hasenfuß, Gerd ; Schaefer, Katrin 
Abstract
Activated platelets secrete different chemokines, among others CCL5, thereby triggering inflammatory cell recruitment into the vessel wall. Here, we investigated how CCL5 deficiency influences vascular remodeling processes. Experiments were performed in apolipoprotein E and CCL5 double deficient (ApoE(-/-) x CCL5(-/-)) mice, using ApoE(-/-) x CCL5(+/+) mice as controls. The ferric chloride model was applied to induce thrombosis at the site of carotid artery injury within minutes and the formation of a smooth muscle cell-rich neointima within 3 weeks. In both groups, vascular injury resulted in thrombus formation. CCL5 deficiency did not alter thrombus resolution examined at day 7. Analysis at 21 days revealed that CCL5 absence was associated with a significant reduction in the neointima area (p < 0.05), neointima-to-media ratio (p < 0.05) and lumen stenosis (p < 0.05) compared to ApoE(-/-) x CCL5(+/+) mice. Immunohistochemical analysis of CCL5 receptors showed decreased CCR5 positive staining in ApoE(-/-) x CCL5(-/-) mice (p < 0.01), whereas the amount of CCR1 (p = 0.053) and Mac2-positive macrophages (p < 0.05) was increased. The amount of SMA-positive smooth muscle cells was lower in ApoE(-/-) mice lacking CCL5 (p < 0.05). Positive staining for Kruppel-like factor 4 (KLF4), an atheroprotective transcription factor, was increased in the neointima of ApoE(-/-) x CCL5(-/-) mice (p < 0.05) and found to co-localize with smooth muscle cells. In summary, CCL5 deficiency resulted in reduced neointima formation after carotid artery injury and thrombosis. Hemodynamic and histochemical analyses suggested that this was not due to differences in thrombus formation or resolution. Possibly, the atheroprotective effect of CCL5 deficiency is mediated by KLF4 upregulation in smooth muscle cells. (C) 2016 Elsevier Ltd. All rights reserved.
Issue Date
2016
Publisher
Pergamon-elsevier Science Ltd
Journal
Thrombosis Research 
ISSN
0049-3848

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