Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.
2017-09-21 | journal article. A publication with affiliation to the University of Göttingen.
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Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs.
Kliesch, T.-T. ; Dietz, J.; Turco, L.; Halder, P.; Polo, E.; Tarantola, M. & Jahn, R. et al. (2017)
Scientific reports, 7(1) art. 12070. DOI: https://doi.org/10.1038/s41598-017-12348-w
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Details
- Authors
- Kliesch, Torben-Tobias ; Dietz, Jörn; Turco, Laura; Halder, Partho; Polo, Elena; Tarantola, Marco ; Jahn, Reinhard ; Janshoff, Andreas
- Abstract
- The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m(-1), at which the number of fusion events is increased substantially.
- Issue Date
- 21-September-2017
- Journal
- Scientific reports
- ISSN
- 2045-2322
- Language
- English
- Sponsor
- Open-Access-Publikationsfonds 2017