X10 expansion microscopy enables 25-nm resolution on conventional microscopes

2018 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​X10 expansion microscopy enables 25-nm resolution on conventional microscopes​
Truckenbrodt, S.; Maidorn, M.; Crzan, D.; Wildhagen, H.; Kabatas, S. & Rizzoli, S. O. ​ (2018) 
EMBO Reports19(9) art. e45836​.​ DOI: https://doi.org/10.15252/embr.201845836 

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Authors
Truckenbrodt, Sven; Maidorn, Manuel; Crzan, Dagmar; Wildhagen, Hanna; Kabatas, Selda; Rizzoli, Silvio O. 
Abstract
Expansion microscopy is a recently introduced imaging technique that achieves super-resolution through physically expanding the specimen by ~4×, after embedding into a swellable gel. The resolution attained is, correspondingly, approximately fourfold better than the diffraction limit, or ~70 nm. This is a major improvement over conventional microscopy, but still lags behind modern STED or STORM setups, whose resolution can reach 20-30 nm. We addressed this issue here by introducing an improved gel recipe that enables an expansion factor of ~10× in each dimension, which corresponds to an expansion of the sample volume by more than 1,000-fold. Our protocol, which we termed X10 microscopy, achieves a resolution of 25-30 nm on conventional epifluorescence microscopes. X10 provides multi-color images similar or even superior to those produced with more challenging methods, such as STED, STORM, and iterative expansion microscopy (iExM). X10 is therefore the cheapest and easiest option for high-quality super-resolution imaging currently available. X10 should be usable in any laboratory, irrespective of the machinery owned or of the technical knowledge.
Issue Date
2018
Journal
EMBO Reports 
Project
info:eu-repo/grantAgreement/EC/FP7/614765/EU//NEUROMOLANATOMY
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | Z03: Unkomplizierte multispektrale, superauflösende Bildgebung durch zehnfache Expansionsmikroskopie 
Organization
Institut für Neuro- und Sinnesphysiologie ; Center for Biostructural Imaging of Neurodegeneration ; DFG Forschungszentrum Molekularphysiologie des Gehirns und Exzellenzcluster Mikroskopie im Nanometerbereich 
Working Group
RG Rizzoli (Quantitative Synaptology in Space and Time) 
ISSN
1469-3178
Language
English

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