RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes

2018 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​RNA helicases mediate structural transitions and compositional changes in pre-ribosomal complexes​
Brüning, L.; Hackert, P.; Martin, R.; Davila Gallesio, J.; Aquino, G. R. R.; Urlaub, H.   & Sloan, K. E. et al.​ (2018) 
Nature Communications9(1) art. 5383​.​ DOI: https://doi.org/10.1038/s41467-018-07783-w 

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Authors
Brüning, Lukas; Hackert, Philipp; Martin, Roman; Davila Gallesio, Jimena; Aquino, Gerald Ryan R.; Urlaub, Henning ; Sloan, Katherine E.; Bohnsack, Markus T. 
Abstract
Production of eukaryotic ribosomal subunits is a highly dynamic process; pre-ribosomes undergo numerous structural rearrangements that establish the architecture present in mature complexes and serve as key checkpoints, ensuring the fidelity of ribosome assembly. Using in vivo crosslinking, we here identify the pre-ribosomal binding sites of three RNA helicases. Our data support roles for Has1 in triggering release of the U14 snoRNP, a critical event during early 40S maturation, and in driving assembly of domain I of pre-60S complexes. Binding of Mak5 to domain II of pre-60S complexes promotes recruitment of the ribosomal protein Rpl10, which is necessary for subunit joining and ribosome function. Spb4 binds to a molecular hinge at the base of ES27 facilitating binding of the export factor Arx1, thereby promoting pre-60S export competence. Our data provide important insights into the driving forces behind key structural remodelling events during ribosomal subunit assembly.
Issue Date
2018
Journal
Nature Communications 
Project
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P14: Die Rolle humaner Nucleoporine in Biogenese und Export makromolekularer Komplexe 
Organization
Institut für Molekularbiologie ; Institut für Klinische Chemie ; Max-Planck-Institut für Biophysikalische Chemie ; Göttinger Zentrum für Molekulare Biowissenschaften 
Working Group
RG M. Bohnsack (Molecular Biology) 
RG Urlaub (Bioanalytische Massenspektrometrie) 
Language
English

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