Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes

2019-12-12 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to: Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Structural Basis of Poxvirus Transcription: Vaccinia RNA Polymerase Complexes​
Grimm, C.; Hillen, H. S. ; Bedenk, K.; Bartuli, J.; Neyer, S.; Zhang, Q. & Hüttenhofer, A. et al.​ (2019) 
Cell179(7) pp. 1537​-1550​.​ DOI: https://doi.org/10.1016/j.cell.2019.11.024 

Documents & Media

License

GRO License GRO License

Details

Authors
Grimm, Clemens; Hillen, Hauke S. ; Bedenk, Kristina; Bartuli, Julia; Neyer, Simon; Zhang, Qian; Hüttenhofer, Alexander; Erlacher, Matthias; Dienemann, Christian ; Schlosser, Andreas; Urlaub, Henning ; Böttcher, Bettina; Szalay, Aladar A; Cramer, Patrick ; Fischer, Utz
Abstract
Poxviruses encode a multisubunit DNA-dependent RNA polymerase (vRNAP) that carries out viral gene expression in the host cytoplasm. We report cryo-EM structures of core and complete vRNAP enzymes from Vaccinia virus at 2.8 Å resolution. The vRNAP core enzyme resembles eukaryotic RNA polymerase II (Pol II) but also reveals many virus-specific features, including the transcription factor Rap94. The complete enzyme additionally contains the transcription factor VETF, the mRNA processing factors VTF/CE and NPH-I, the viral core protein E11, and host tRNAGln. This complex can carry out the entire early transcription cycle. The structures show that Rap94 partially resembles the Pol II initiation factor TFIIB, that the vRNAP subunit Rpo30 resembles the Pol II elongation factor TFIIS, and that NPH-I resembles chromatin remodeling enzymes. Together with the accompanying paper (Hillen et al., 2019), these results provide the basis for unraveling the mechanisms of poxvirus transcription and RNA processing.
Issue Date
12-December-2019
Journal
Cell 
Project
EXC 2067: Multiscale Bioimaging 
Working Group
RG Cramer 
RG Hillen (Structure and Function of Molecular Machines) 
ISSN
0092-8674
eISSN
1097-4172
Language
English
Notes
Research funded by Deutsche Forschungsgemeinschaft (SPP193518-1SFB860Fi 573 7-2) | European Research Council (693023) | Volkswagen Foundation | Austrian Science Fund (P-30486-BBLSFB F4411) | Germany’s Excellence Strategy (EXC 2067/1- 390729940) | Genelux Corporation

Reference

Citations


Social Media