Vesicle adhesion in the electrostatic strong-coupling regime studied by time-resolved small-angle X-ray scattering
2020-05-07 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Vesicle adhesion in the electrostatic strong-coupling regime studied by time-resolved small-angle X-ray scattering
Komorowski, K. ; Sztucki, M.; Sharpnack, L.; Brehm, G. ; Köster, S. ; Salditt, T. & Schaeper, J. (2020)
Soft Matter, 16(17) pp. 4142-4154. DOI: https://doi.org/10.1039/D0SM00259C
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- Authors
- Komorowski, Karlo ; Sztucki, Michael; Sharpnack, Lewis; Brehm, Gerrit ; Köster, Sarah ; Salditt, Tim ; Schaeper, Jannis
- Abstract
- We have used time-resolved small-angle X-ray scattering (SAXS) to study the adhesion of lipid vesicles in the electrostatic strong-coupling regime induced by divalent ions. The bilayer structure and the interbilayer distance dw between adhered vesicles was studied for different DOPC:DOPS mixtures varying the surface charge density of the membrane, as well as for different divalent ions, such as Ca2+, Sr2+, and Zn2+. The results are in good agreement with the strong coupling theory predicting the adhesion state and the corresponding like-charge attraction based on ion-correlations. Using SAXS combined with the stopped-flow rapid mixing technique, we find that in highly charged bilayers the adhesion state is only of transient nature, and that the adhering vesicles subsequently transform to a phase of multilamellar vesicles, again with an inter-bilayer distance according to the theory of strong binding. Aside from the stopped-flow SAXS instrumentations used primarily for these results, we also evaluate microfluidic sample environments for vesicle SAXS in view of future extension of this work.
- Issue Date
- 7-May-2020
- Journal
- Soft Matter
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1286: Quantitative Synaptologie
SFB 1286 | A02: Bestimmung der Struktur synaptischer Organellen durch Röntgenbeugungs- und Bildgebungsverfahren
SFB 1286 | B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters - Organization
- Institut für Röntgenphysik
- Working Group
- RG Köster (Cellular Biophysics)
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) - ISSN
- 1744-683X
- eISSN
- 1744-6848
- Language
- English
- Subject(s)
- x-ray scattering; membrane biophysics; microfluidics