Load-Induced Cardiomyocyte Apoptosis in Cultured Multicellular Myocardial Preparations Is Unaltered in Presence of the beta-Adrenoceptor Antagonist Nebivolol
2009 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Load-Induced Cardiomyocyte Apoptosis in Cultured Multicellular Myocardial Preparations Is Unaltered in Presence of the beta-Adrenoceptor Antagonist Nebivolol
Janssen, P. M. L.; Zeitz, O.; Schumann, H.; Holtz, J. & Hasenfuß, G. (2009)
Pharmacology, 83(3) pp. 141-147. DOI: https://doi.org/10.1159/000187428
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- Authors
- Janssen, P. M. L.; Zeitz, Oliver; Schumann, H.; Holtz, J.; Hasenfuß, Gerd
- Abstract
- Overload-induced heart failure is associated with enhanced apoptosis of cardiomyocytes, and increased mechanical load is an inductor of this apoptosis. It is unknown whether nebivolol, a third generation beta(1)-adrenoceptor antagonist, possesses properties that can attenuate this apoptosis. Multicellular preparations from rabbit hearts were mounted in a culture system that allows for measurement of contractile parameters over several days. Culturing these muscles on a constant high preload induces apoptosis of the cardiomyocytes. Of each heart, 1 preloaded muscle preparation was treated with nebivolol (10(-6) mol/l), 1 preloaded without continuous exposure to nebivolol (positive control) and 1 unloaded (negative control). After 48 h of continuous loaded contractions, apoptosis was assessed by TUNEL-assay to confirm that nuclei of myocytes were affected, or by DNA-ladder intensity analysis for semiquantification. Maximal twitch force development was slightly, but significantly, lower in preparations contracting in presence of nebivolol (compared to solvent) while twitch-timing parameters were similar. After 48 h of continuous contractions, no additional differences were observed between the groups regarding contractile parameters. DNA-ladder analysis showed a similar rate of apoptosis in presence of nebivolol. Nebivolol does not increase, nor decrease, the rate of load-induced cardiomyocyte apoptosis. Copyright (C) 2008 S. Karger AG, Basel
- Issue Date
- 2009
- Publisher
- Karger
- Journal
- Pharmacology
- ISSN
- 0031-7012