Trophic and Non-Trophic Interactions in a Biodiversity Experiment Assessed by Next-Generation Sequencing

2016 | journal article. A publication with affiliation to the University of Göttingen.

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​Trophic and Non-Trophic Interactions in a Biodiversity Experiment Assessed by Next-Generation Sequencing​
Tiede, J. ; Wemheuer, B. ; Traugott, M.; Daniel, R. ; Tscharntke, T. ; Ebeling, A. & Scherber, C. ​ (2016) 
PLOS ONE11(2) art. e0148781​.​ DOI: https://doi.org/10.1371/journal.pone.0148781 

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Authors
Tiede, Julia ; Wemheuer, Bernd ; Traugott, Michael; Daniel, Rolf ; Tscharntke, Teja ; Ebeling, Anne; Scherber, Christoph 
Abstract
Plant diversity affects species richness and abundance of taxa at higher trophic levels. However, plant diversity effects on omnivores (feeding on multiple trophic levels) and their trophic and non-trophic interactions are not yet studied because appropriate methods were lacking. A promising approach is the DNA-based analysis of gut contents using next generation sequencing (NGS) technologies. Here, we integrate NGS-based analysis into the framework of a biodiversity experiment where plant taxonomic and functional diversity were manipulated to directly assess environmental interactions involving the omnivorous ground beetle Pterostichus melanarius. Beetle regurgitates were used for NGS-based analysis with universal 18S rDNA primers for eukaryotes. We detected a wide range of taxa with the NGS approach in regurgitates, including organisms representing trophic, phoretic, parasitic, and neutral interactions with P. melanarius. Our findings suggest that the frequency of (i) trophic interactions increased with plant diversity and vegetation cover; (ii) intraguild predation increased with vegetation cover, and (iii) neutral interactions with organisms such as fungi and protists increased with vegetation cover. Experimentally manipulated plant diversity likely affects multitrophic interactions involving omnivorous consumers. Our study therefore shows that trophic and non-trophic interactions can be assessed via NGS to address fundamental questions in biodiversity research.
Issue Date
2016
Journal
PLOS ONE 
Organization
Fakultät für Agrarwissenschaften ; Department für Nutzpflanzenwissenschaften ; Abteilung Agrarökologie 
ISSN
1932-6203
Language
English
Sponsor
Open-Access Publikationsfonds 2016

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