Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry
2019 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Fluorinated nanobodies for targeted molecular imaging of biological samples using nanoscale secondary ion mass spectrometry
Kabatas, S. ; Agüi-Gonzalez, P. ; Hinrichs, R. ; Jähne, S. ; Opazo, F. ; Diederichsen, U. & Rizzoli, S. O. et al. (2019)
Journal of Analytical Atomic Spectrometry, 34(6) pp. 1083-1087. DOI: https://doi.org/10.1039/C9JA00117D
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Details
- Authors
- Kabatas, Selda ; Agüi-Gonzalez, Paola ; Hinrichs, Rena ; Jähne, Sebastian ; Opazo, Felipe ; Diederichsen, Ulf ; Rizzoli, Silvio O. ; Phan, Nhu T. N.
- Abstract
- Molecular imaging of targeted large biomolecules has been restricted in SIMS due to the limited number of probes containing SIMSdetectable isotopes.We introduce here new 19F-containingmolecules that can be conjugated in a site-specificmanner to nanobodies able to recognize fluorescent proteins (FPs) or mouse immunoglobulins (Igs). In this work, we demonstrate that it is possible to use the 19F-nanobodies to reveal the location of several cellular proteins previously tagged with FPs or Igs. This enables specific bio-imaging in SIMS for a vast repertoire of biomolecules, offering new opportunities to study specific structural and functional molecular interactions in biological specimens.
- Issue Date
- 2019
- Journal
- Journal of Analytical Atomic Spectrometry
- Project
- SFB 1286: Quantitative Synaptologie
SFB 1286 | B01: Der Verteilung struktureller Lipide in synaptischen Membranen - Working Group
- RG Phan
RG Rizzoli (Quantitative Synaptology in Space and Time) - ISSN
- 0267-9477; 1364-5544
- eISSN
- 1364-5544
- Language
- English
