Dynamic instability of clathrin assembly provides proofreading control for endocytosis
2019 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Dynamic instability of clathrin assembly provides proofreading control for endocytosis
Chen, Y.; Yong, J.; Martínez-Sánchez, A.; Yang, Y.; Wu, Y.; De Camilli, P. & Fernández Busnadiego, R. et al. (2019)
Journal of Cell Biology, 218(10) pp. 3200-3211. DOI: https://doi.org/10.1083/jcb.201804136
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Details
- Authors
- Chen, Yan; Yong, Jeffery; Martínez-Sánchez, Antonio; Yang, Yang; Wu, Yumei; De Camilli, Pietro; Fernández Busnadiego, Rubén ; Wu, Min
- Abstract
- Clathrin-mediated endocytosis depends on the formation of functional clathrin-coated pits that recruit cargos and mediate the uptake of those cargos into the cell. However, it remains unclear whether the cargos in the growing clathrin-coated pits are actively monitored by the coat assembly machinery. Using a cell-free reconstitution system, we report that clathrin coat formation and cargo sorting can be uncoupled, indicating that a checkpoint is required for functional cargo incorporation. We demonstrate that the ATPase Hsc70 and a dynamic exchange of clathrin during assembly are required for this checkpoint. In the absence of Hsc70 function, clathrin assembles into pits but fails to enrich cargo. Using single-molecule imaging, we further show that uncoating takes place throughout the lifetime of the growing clathrin-coated pits. Our results suggest that the dynamic exchange of clathrin, at the cost of the reduced overall assembly rates, primarily serves as a proofreading mechanism for quality control of endocytosis.
- Issue Date
- 2019
- Journal
- Journal of Cell Biology
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Fernández-Busnadiego (Structural Cell Biology)
- Language
- English