A comparative analysis of the mobility of 45 proteins in the synaptic bouton
2020-08-17 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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- Authors
- Reshetniak, Sofiia; Ußling, Jan‐Eike; Perego, Eleonora; Rammner, Burkhard; Schikorski, Thomas; Fornasiero, Eugenio F. ; Truckenbrodt, Sven; Köster, Sarah ; Rizzoli, Silvio O.
- Abstract
- Abstract Many proteins involved in synaptic transmission are well known, and their features, as their abundance or spatial distribution, have been analyzed in systematic studies. This has not been the case, however, for their mobility. To solve this, we analyzed the motion of 45 GFP‐tagged synaptic proteins expressed in cultured hippocampal neurons, using fluorescence recovery after photobleaching, particle tracking, and modeling. We compared synaptic vesicle proteins, endo‐ and exocytosis cofactors, cytoskeleton components, and trafficking proteins. We found that movement was influenced by the protein association with synaptic vesicles, especially for membrane proteins. Surprisingly, protein mobility also correlated significantly with parameters as the protein lifetimes, or the nucleotide composition of their mRNAs. We then analyzed protein movement thoroughly, taking into account the spatial characteristics of the system. This resulted in a first visualization of overall protein motion in the synapse, which should enable future modeling studies of synaptic physiology.
Synopsis image Live imaging reveals global protein dynamics in the synaptic bouton, providing a first visualization of overall protein motion in the synapse and showing connections between various protein characteristics and mobility in vivo. Membrane proteins display lower mobility rates than soluble proteins. Proteins are less mobile in synaptic boutons than in axons. Protein mobility is strongly influenced by the protein association with synaptic vesicles. Amino acid composition, mRNA nucleotide composition, and protein lifetimes correlate with mobility parameters. Provided diffusion coefficients for 45 synaptic proteins can be used to generate models of synaptic physiology.
Live imaging reveals global protein dynamics in the synaptic bouton, providing a first visualization of overall protein motion in the synapse and showing connections between protein characteristics and mobility in vivo. image - Issue Date
- 17-August-2020
- Journal
- The EMBO Journal
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente
SFB 1190 | P09: Proteinsortierung in der Synapse: Prinzipien und molekulare Organisation
SFB 1286: Quantitative Synaptologie
SFB 1286 | B02: Ein in vitro-Verfahren zum Verständnis der struktur-organisierenden Rolle des Vesikel-Clusters
SFB 1286 | Z02: Integrative Datenanalyse und -interpretation. Generierung einer synaptisch-integrativen Datenstrategie (SynIDs) - Organization
- Institut für Röntgenphysik
- Working Group
- RG Köster (Cellular Biophysics)
RG Rizzoli (Quantitative Synaptology in Space and Time)
RG Bonn - ISSN
- 0261-4189
- eISSN
- 1460-2075
- Language
- English
- Subject(s)
- neuro biophysics; molecular biophysics; cellular biophysics
- Sponsor
- European Research Council http://dx.doi.org/10.13039/100010663
Germany's Excellence Strategy
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
