3d phase‐contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects

2020-10-06 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​3d phase‐contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects​
Eckermann, M.; Peruzzi, N.; Frohn, J. ; Bech, M.; Englund, E.; Veress, B. & Salditt, T.  et al.​ (2020) 
Skin Research & Technology,.​ DOI: https://doi.org/10.1111/srt.12974 

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Authors
Eckermann, Marina; Peruzzi, Niccolò; Frohn, Jasper ; Bech, Martin; Englund, Elisabet; Veress, Béla; Salditt, Tim ; Dahlin, Lars B.; Ohlsson, Bodil
Abstract
BackgroundEnteric neuropathy is described in most patients with gastrointestinal dysmotility and may be found together with reduced intraepidermal nerve fiber density (IENFD). The aim of this pilot study was to assess whether three-dimensional (3d) imaging of skin biopsies could be used to examine various tissue components in patients with gastrointestinal dysmotility.Material and methodsFour dysmotility patients of different etiology and two healthy volunteers were included. From each subject, two 3-mm punch skin biopsies were stained with antibodies against protein gene product 9.5 or evaluated as a whole with two X-ray phase-contrast computed tomography (CT) setups, a laboratory µCT setup and a dedicated synchrotron radiation nanoCT end-station.ResultsTwo patients had reduced IENFD, and two normal IENFD, compared with controls. µCT and X-ray phase-contrast holographic nanotomography scanned whole tissue specimens, with optional high-resolution scans revealing delicate structures, without differentiation of various fibers and cells. Irregular architecture of dermal fibers was observed in the patient with Ehlers-Danlos syndrome and the patient with idiopathic dysmotility showed an abundance of mesenchymal ground substance.Conclusions3d phase-contrast tomographic imaging may be useful to illustrate traits of connective tissue dysfunction in various organs and to demonstrate whether disorganized dermal fibers could explain organ dysfunction.
Issue Date
6-October-2020
Journal
Skin Research & Technology 
Project
EXC 2067: Multiscale Bioimaging 
Organization
Institut für Röntgenphysik 
Working Group
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) 
ISSN
0909-752X
eISSN
1600-0846
Language
English
Subject(s)
biomedical tomography

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