X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction

2020-10-06 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​X-Ray Structural Analysis of Single Adult Cardiomyocytes: Tomographic Imaging and Microdiffraction​
Reichardt, M.; Neuhaus, C.; Nicolas, J.-D.; Bernhardt, M.; Toischer, K.   & Salditt, T. ​ (2020) 
Biophysical Journal119(7) pp. 1309​-1323​.​ DOI: https://doi.org/10.1016/j.bpj.2020.08.019 

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Authors
Reichardt, Marius; Neuhaus, Charlotte; Nicolas, Jan-David; Bernhardt, Marten; Toischer, Karl ; Salditt, Tim 
Abstract
We present a multiscale imaging approach to characterize the structure of isolated adult murine cardiomyocytes based on a combination of full-field three-dimensional coherent x-ray imaging and scanning x-ray diffraction. Using these modalities, we probe the structure from the molecular to the cellular scale. Holographic projection images on freeze-dried cells have been recorded using highly coherent and divergent x-ray waveguide radiation. Phase retrieval and tomographic reconstruction then yield the three-dimensional electron density distribution with a voxel size below 50 nm. In the reconstruction volume, myofibrils, sarcomeric organization, and mitochondria can be visualized and quantified within a single cell without sectioning. Next, we use microfocusing optics by compound refractive lenses to probe the diffraction signal of the actomyosin lattice. Comparison between recordings of chemically fixed and untreated, living cells indicate that the characteristic lattice distances shrink by ∼10% upon fixation.
Issue Date
6-October-2020
Journal
Biophysical Journal 
Project
EXC 2067: Multiscale Bioimaging 
Organization
Institut für Röntgenphysik 
Working Group
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) 
ISSN
0006-3495
Language
English
Subject(s)
x-ray imaging; x-ray scattering; biomedical tomography

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