A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​A 20‐kb lineage‐specific genomic region tames virulence in pathogenic amphidiploid Verticillium longisporum​
Harting, R.; Starke, J.; Kusch, H. ; Pöggeler, S.; Maurus, I.; Schlüter, R. & Landesfeind, M. et al.​ (2021) 
Molecular Plant Pathology,.​ DOI: https://doi.org/10.1111/mpp.13071 

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Authors
Harting, Rebekka; Starke, Jessica; Kusch, Harald ; Pöggeler, Stefanie; Maurus, Isabel; Schlüter, Rabea; Landesfeind, Manuel; Bulla, Ingo; Nowrousian, Minou; Braus, Gerhard H.; de Jonge, Ronnie; Stahlhut, Gertrud; Hoff, Katharina J.; Aßhauer, Kathrin P.; Thürmer, Andrea; Stanke, Mario; Daniel, Rolf; Morgenstern, Burkhard ; Thomma, Bart P. H. J.; Kronstad, James W.; Braus‐Stromeyer, Susanna A.
Abstract
Abstract Amphidiploid fungal Verticillium longisporum strains Vl43 and Vl32 colonize the plant host Brassica napus but differ in their ability to cause disease symptoms. These strains represent two V. longisporum lineages derived from different hybridization events of haploid parental Verticillium strains. Vl32 and Vl43 carry same‐sex mating‐type genes derived from both parental lineages. Vl32 and Vl43 similarly colonize and penetrate plant roots, but asymptomatic Vl32 proliferation in planta is lower than virulent Vl43. The highly conserved Vl43 and Vl32 genomes include less than 1% unique genes, and the karyotypes of 15 or 16 chromosomes display changed genetic synteny due to substantial genomic reshuffling. A 20 kb Vl43 lineage‐specific (LS) region apparently originating from the Verticillium dahliae‐related ancestor is specific for symptomatic Vl43 and encodes seven genes, including two putative transcription factors. Either partial or complete deletion of this LS region in Vl43 did not reduce virulence but led to induction of even more severe disease symptoms in rapeseed. This suggests that the LS insertion in the genome of symptomatic V. longisporum Vl43 mediates virulence‐reducing functions, limits damage on the host plant, and therefore tames Vl43 from being even more virulent.
A lineage‐specific region in the Verticillium longisporum Vl43 genome reduces fungal virulence of infected rapeseed host plants. image
Issue Date
2021
Journal
Molecular Plant Pathology 
Organization
Abteilung Molekulare Mikrobiologie & Genetik 
ISSN
1464-6722
eISSN
1364-3703
Language
English
Sponsor
Bundesministerium für Bildung und Forschung: BioFung http://dx.doi.org/10.13039/501100002347
Natural Sciences and Engineering Research Council of Canada: CREATE http://dx.doi.org/10.13039/501100000038
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Open-Access-Publikationsfonds 2021

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