Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex

2021 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Molecular Profiling Reveals Involvement of ESCO2 in Intermediate Progenitor Cell Maintenance in the Developing Mouse Cortex​
Ulmke, P. A.; Sakib, M. S.; Ditte, P.; Sokpor, G. ; Kerimoglu, C. ; Pham, L. & Xie, Y. et al.​ (2021) 
Stem Cell Reports16(4) pp. 968​-984​.​ DOI: https://doi.org/10.1016/j.stemcr.2021.03.008 

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Authors
Ulmke, Pauline Antonie; Sakib, M. Sadman; Ditte, Peter; Sokpor, Godwin ; Kerimoglu, Cemil ; Pham, Linh; Xie, Yuanbin; Mao, Xiaoyi; Rosenbusch, Joachim ; Tuoc, Tran 
Abstract
Intermediate progenitor cells (IPCs) are neocortical neuronal precursors. Although IPCs play crucial roles in corticogenesis, their molecular features remain largely unknown. In this study, we aimed to characterize the molecular profile of IPCs. We isolated TBR2-positive (+) IPCs and TBR2-negative (−) cell populations in the developing mouse cortex. Comparative genome-wide gene expression analysis of TBR2+ IPCs versus TBR2− cells revealed differences in key factors involved in chromatid segregation, cell-cycle regulation, transcriptional regulation, and cell signaling. Notably, mutation of many IPC genes in human has led to intellectual disability and caused a wide range of cortical malformations, including microcephaly and agenesis of corpus callosum. Loss-of-function experiments in cortex-specific mutants of Esco2, one of the novel IPC genes, demonstrate its critical role in IPC maintenance, and substantiate the identification of a central genetic determinant of IPC biogenesis. Our data provide novel molecular characteristics of IPCs in the developing mouse cortex.
Issue Date
2021
Journal
Stem Cell Reports 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | B06: Die Rolle von RNA in Synapsenphysiologie und Neurodegeneration 
Working Group
RG A. Fischer (Epigenetics and Systems Medicine in Neurodegenerative Diseases) 
ISSN
2213-6711
Language
English

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