Multiscale photonic imaging of the native and implanted cochlea

2021-05-04 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Multiscale photonic imaging of the native and implanted cochlea​
Keppeler, D.; Kampshoff, C. A.; Thirumalai, A.; Duque-Afonso, C. J.; Schaeper, J. J. ; Quilitz, T. & Töpperwien, M.  et al.​ (2021) 
Proceedings of the National Academy of Sciences of the United States of America118(18) art. e2014472118​.​ DOI: https://doi.org/10.1073/pnas.2014472118 

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Authors
Keppeler, Daniel; Kampshoff, Christoph A.; Thirumalai, Anupriya; Duque-Afonso, Carlos J.; Schaeper, Jannis J. ; Quilitz, Tabea; Töpperwien, Mareike ; Vogl, Christian; Hessler, Roland; Meyer, Alexander; Salditt, Tim ; Moser, Tobias 
Abstract
The cochlea of our auditory system is an intricate structure deeply embedded in the temporal bone. Compared with other sensory organs such as the eye, the cochlea has remained poorly accessible for investigation, for example, by imaging. This limitation also concerns the further development of technology for restoring hearing in the case of cochlear dysfunction, which requires quantitative information on spatial dimensions and the sensorineural status of the cochlea. Here, we employed X-ray phase-contrast tomography and light-sheet fluorescence microscopy and their combination for multiscale and multimodal imaging of cochlear morphology in species that serve as established animal models for auditory research. We provide a systematic reference for morphological parameters relevant for cochlear implant development for rodent and nonhuman primate models. We simulate the spread of light from the emitters of the optical implants within the reconstructed nonhuman primate cochlea, which indicates a spatially narrow optogenetic excitation of spiral ganglion neurons.
Issue Date
4-May-2021
Journal
Proceedings of the National Academy of Sciences of the United States of America 
Project
EXC 2067: Multiscale Bioimaging 
Organization
Institut für Röntgenphysik 
Working Group
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) 
RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding) 
ISSN
0027-8424
eISSN
1091-6490
Language
English
Subject(s)
x-ray imaging; biomedical tomography

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