Measurement of Angstrom to Nanometer Molecular Distances with 19 F Nuclear Spins by EPR/ENDOR Spectroscopy
2019 | journal article. A publication with affiliation to the University of Göttingen.
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Measurement of Angstrom to Nanometer Molecular Distances with 19 F Nuclear Spins by EPR/ENDOR Spectroscopy
Meyer, A.; Dechert, S.; Dey, S.; Höbartner, C. & Bennati, M. (2019)
Angewandte Chemie. International Edition, 59(58) pp. 373-379. DOI: https://doi.org/10.1002/anie.201908584
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Details
- Authors
- Meyer, Andreas; Dechert, Sebastian; Dey, Surjendu; Höbartner, Claudia; Bennati, Marina
- Abstract
- Spectroscopic and biophysical methods for structural determination at atomic resolution are fundamental in studies of biological function. Here we introduce an approach to measure molecular distances in bio-macromolecules using 19 F nuclear spins and nitroxide radicals in combination with high-frequency (94 GHz/3.4 T) electron-nuclear double resonance (ENDOR). The small size and large gyromagnetic ratio of the 19 F label enables to access distances up to about 1.5 nm with an accuracy of 0.1-1 Å. The experiment is not limited by the size of the bio-macromolecule. Performance is illustrated on synthesized fluorinated model compounds as well as spin-labelled RNA duplexes. The results demonstrate that our simple but strategic spin-labelling procedure combined with state-of-the-art spectroscopy accesses a distance range crucial to elucidate active sites of nucleic acids or proteins in the solution state.
- Issue Date
- 2019
- Journal
- Angewandte Chemie. International Edition
- Organization
- Fakultät für Chemie
- ISBN
- 31539187
- ISSN
- 1521-3773
- eISSN
- 1521-3773
- Language
- English