Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer

2021-12-22 | preprint. A publication with affiliation to the University of Göttingen.

Jump to: Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer​
Thiele, J. C. ; Jungblut, M.; Helmerich, D. A.; Tsukanov, R. ; Chizhik, A. M. ; Chizhik, A. I.  & Schnermann, M. et al.​ (2021). DOI: https://doi.org/10.1101/2021.12.20.473473 

Documents & Media

License

GRO License GRO License

Details

Authors
Thiele, Jan Christoph ; Jungblut, Marvin; Helmerich, Dominic A.; Tsukanov, Roman ; Chizhik, Anna M. ; Chizhik, Alexey I. ; Schnermann, Martin; Sauer, Markus; Nevskyi, Oleksii; Enderlein, Jörg 
Abstract
Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously.
Issue Date
22-December-2021
Project
EXC 2067: Multiscale Bioimaging 
Organization
III. Physikalisches Institut - Biophysik 
Working Group
RG Enderlein 
Extent
16
Language
English

Reference

Citations


Social Media