Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer
2021-12-22 | preprint. A publication with affiliation to the University of Göttingen.
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- Authors
- Thiele, Jan Christoph ; Jungblut, Marvin; Helmerich, Dominic A.; Tsukanov, Roman ; Chizhik, Anna M. ; Chizhik, Alexey I. ; Schnermann, Martin; Sauer, Markus; Nevskyi, Oleksii; Enderlein, Jörg
- Abstract
- Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously.
- Issue Date
- 22-December-2021
- Project
- EXC 2067: Multiscale Bioimaging
- Organization
- III. Physikalisches Institut - Biophysik
- Working Group
- RG Enderlein
- Extent
- 16
- Language
- English