Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
2022-01-11 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Fluorescence lifetime DNA-PAINT for multiplexed super-resolution imaging of cells
Oleksiievets, N.; Sargsyan, Y.; Thiele, J. C. ; Mougios, N.; Sograte-Idrissi, S.; Nevskyi, O. & Gregor, I. et al. (2022)
Communications Biology, 5(1) pp. 38. DOI: https://doi.org/10.1038/s42003-021-02976-4
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Details
- Authors
- Oleksiievets, Nazar; Sargsyan, Yelena; Thiele, Jan Christoph ; Mougios, Nikolaos; Sograte-Idrissi, Shama; Nevskyi, Oleksii; Gregor, Ingo; Opazo, Felipe; Thoms, Sven ; Enderlein, Jörg ; Tsukanov, Roman
- Abstract
- DNA point accumulation for imaging in nanoscale topography (DNA-PAINT) is a powerful super-resolution technique highly suitable for multi-target (multiplexing) bio-imaging. However, multiplexed imaging of cells is still challenging due to the dense and sticky environment inside a cell. Here, we combine fluorescence lifetime imaging microscopy (FLIM) with DNA-PAINT and use the lifetime information as a multiplexing parameter for targets identification. In contrast to Exchange-PAINT, fluorescence lifetime PAINT (FL-PAINT) can image multiple targets simultaneously and does not require any fluid exchange, thus leaving the sample undisturbed and making the use of flow chambers/microfluidic systems unnecessary. We demonstrate the potential of FL-PAINT by simultaneous imaging of up to three targets in a cell using both wide-field FLIM and 3D time-resolved confocal laser scanning microscopy (CLSM). FL-PAINT can be readily combined with other existing techniques of multiplexed imaging and is therefore a perfect candidate for high-throughput multi-target bio-imaging.
- Issue Date
- 11-January-2022
- Journal
- Communications Biology
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz
SFB 1002 | A10: Peroxisomen als modulatorische Einheiten im Herzstoffwechsel und bei Herzinsuffizienz - Organization
- III. Physikalisches Institut - Biophysik
- Working Group
- RG Enderlein
RG Thoms (Biochemistry and Molecular Medicine) - ISSN
- 2399-3642
- Language
- English
- Sponsor
- Open-Access-Publikationsfonds 2022