Allosteric transcription stimulation by RNA polymerase II super elongation complex

2021 | journal article; research paper

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​Chen Y, Vos SM, Dienemann C, Ninov M, Urlaub H, Cramer P. ​Allosteric transcription stimulation by RNA polymerase II super elongation complex​. ​​Molecular Cell. ​2021;​81​(16):​​3386.e10​-3399.e10​. ​doi:10.1016/j.molcel.2021.06.019. 

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Authors
Chen, Ying; Vos, Seychelle M.; Dienemann, Christian; Ninov, Momchil; Urlaub, Henning; Cramer, Patrick 
Abstract
The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we report the cryoelectron microscopy (cryo-EM) structure of ELL2-EAF1 bound to a RNA Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the RNA Pol II lobe domain, explaining how SEC delivers P-TEFb to RNA Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that the stimulation of RNA elongation requires the dimerization module and the ELL2 linker that tethers the module to the RNA Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a closed conformation of the RNA Pol II active center cleft.
Issue Date
2021
Journal
Molecular Cell 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | A06: Mitochondrienfunktion und -umsatz in Synapsen 
Working Group
RG Cramer 
RG Urlaub (Bioanalytische Massenspektrometrie) 
External URL
https://mbexc.uni-goettingen.de/literature/publications/326
https://sfb1286.uni-goettingen.de/literature/publications/127
ISSN
1097-2765
eISSN
1097-4164
Language
English

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