Mechanism of spindle pole organization and instability in human oocytes

2022 | Zeitschriftenartikel; Forschungsarbeit

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​Mechanism of spindle pole organization and instability in human oocytes​
So, C.; Menelaou, K.; Uraji, J.; Harasimov, K.; Steyer, A. M.; Seres, K. B. & Bucevičius, J. u.a.​ (2022) 
Science375(6581) art. eabj3944​.​ DOI: https://doi.org/10.1126/science.abj3944 

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Autor(en)
So, Chun; Menelaou, Katerina; Uraji, Julia; Harasimov, Katarina; Steyer, Anna M.; Seres, K. Bianka; Bucevičius, Jonas; Lukinavičius, Gražvydas; Möbius, Wiebke ; Sibold, Claus; Tandler-Schneider, Andreas; Eckel, Heike; Moltrecht, Rüdiger; Blayney, Martyn; Elder, Kay; Schuh, Melina 
Zusammenfassung
Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)-mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.
Erscheinungsdatum
2022
Zeitschrift
Science 
Project
EXC 2067: Multiscale Bioimaging 
FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala 
FOR 2848 | P08: Strukturelle und funktionale Veränderungen der inneren mitochondrialen Membran axonaler Mitochondrien in vivo in einem dymyelinisierenden Mausmodell 
Arbeitsgruppe
RG Möbius 
RG Schuh 
ISSN
0036-8075
eISSN
1095-9203
Sprache
Englisch

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