Germacrene A Synthases for Sesquiterpene Lactone Biosynthesis Are Expressed in Vascular Parenchyma Cells Neighboring Laticifers in Lettuce

2022-04-28 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Germacrene A Synthases for Sesquiterpene Lactone Biosynthesis Are Expressed in Vascular Parenchyma Cells Neighboring Laticifers in Lettuce​
Kwon, M.; Hodgins, C. L.; Haslam, T. M.; Roth, S. A.; Nguyen, T.-D.; Yeung, E. C. & Ro, D.-K.​ (2022) 
Plants11(9).​ DOI: https://doi.org/10.3390/plants11091192 

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Authors
Kwon, Moonhyuk; Hodgins, Connor L.; Haslam, Tegan M.; Roth, Susan A.; Nguyen, Trinh-Don; Yeung, Edward C.; Ro, Dae-Kyun
Abstract
Sesquiterpene lactone (STL) and natural rubber (NR) are characteristic isoprenoids in lettuce (Lactuca sativa). Both STL and NR co-accumulate in laticifers, pipe-like structures located along the vasculature. NR-biosynthetic genes are exclusively expressed in laticifers, but cell-type specific expression of STL-biosynthetic genes has not been studied. Here, we examined the expression pattern of germacrene A synthase (LsGAS), which catalyzes the first step in STL biosynthesis in lettuce. Quantitative PCR and Illumina read mapping revealed that the transcripts of two GAS isoforms (LsGAS1/LsGAS2) are expressed two orders of magnitude (~100–200) higher in stems than laticifers. This result implies that the cellular site for LsGAS1/2 expression is not in laticifers. To gain more insights, promoters of LsGAS1/2 were cloned and fused to β-glucuronidase (GUS), followed by transformations of lettuce with these promoter-GUS constructs. In in situ GUS assays, the GUS expression driven by the LsGAS1/2 promoters was tightly associated with vascular bundles. High-resolution microsections showed that GUS signals are not present in laticifers but are detected in the vascular parenchyma cells neighboring the laticifers. These results suggest that expression of LsGAS1/2 occurs in the parenchyma cells neighboring laticifers, while the resulting STL metabolites accumulate in laticifers. It can be inferred that active metabolite-trafficking occurs from the parenchyma cells to laticifers in lettuce.
Issue Date
28-April-2022
Journal
Plants 
eISSN
2223-7747
Language
English
Sponsor
Natural Sciences and Engineering Research Council of Canada (NSERC)
National Research Foundation of Korea (NRF)
Ministry of Trade, Industry and Energy (MOTIE, Korea)

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