A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution
2022 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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A fluorescent nanosensor paint detects dopamine release at axonal varicosities with high spatiotemporal resolution
Elizarova, S.; Chouaib, A. A.; Shaib, A.; Hill, B.; Mann, F.; Brose, N. & Kruss, S. et al. (2022)
Proceedings of the National Academy of Sciences, 119(22) art. e2202842119. DOI: https://doi.org/10.1073/pnas.2202842119
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Details
- Authors
- Elizarova, Sofia; Chouaib, Abed Alrahman; Shaib, Ali; Hill, Björn; Mann, Florian; Brose, Nils ; Kruss, Sebastian ; Daniel, James A.
- Abstract
- Significance The neurotransmitter dopamine controls normal behavior and dopaminergic dysfunction is prevalent in multiple brain diseases. To reach a detailed understanding of how dopamine release and signaling are regulated at the subcellular level, we developed a near infrared fluorescent dopamine nanosensor 'paint' (AndromeDA) to directly image dopamine release and its spatiotemporal characteristics. With AndromeDA, we can ascribe discrete DA release events to defined axonal varicosities, directly assess the heterogeneity of DA release events across such release sites, and determine the molecular components of the DA release machinery. AndromeDA thus provides a new method for gaining fundamental insights into the core mechanisms of dopamine release, which with greatly benefit our knowledge of dopamine biology and pathobiology.
- Issue Date
- 2022
- Journal
- Proceedings of the National Academy of Sciences
- Project
- EXC 2067: Multiscale Bioimaging
- Working Group
- RG Brose
- External URL
- https://mbexc.uni-goettingen.de/literature/publications/493
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Language
- English