Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner

2022 | journal article. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Diet triggers specific responses of hypothalamic astrocytes in time and region dependent manner​
Lutomska, L. M.; Miok, V.; Krahmer, N.; González García, I.; Gruber, T.; Le Thuc, O. & Murat, C. D. et al.​ (2022) 
Glia, art. glia.24237​.​ DOI: https://doi.org/10.1002/glia.24237 

Details

Authors
Lutomska, Luiza Maria; Miok, Viktorian; Krahmer, Natalie; González García, Ismael; Gruber, Tim; Le Thuc, Ophélia; Murat, Cahuê DB; Legutko, Beata; Sterr, Michael; Saher, Gesine; García‐Cáceres, Cristina
Abstract
Hypothalamic astrocytes are particularly affected by energy-dense food consumption. How the anatomical location of these glial cells and their spatial molecular distribution in the arcuate nucleus of the hypothalamus (ARC) determine the cellular response to a high caloric diet remains unclear. In this study, we investigated their distinctive molecular responses following exposure to a high-fat high-sugar (HFHS) diet, specifically in the ARC. Using RNA sequencing and proteomics, we showed that astrocytes have a distinct transcriptomic and proteomic profile dependent on their anatomical location, with a major proteomic reprogramming in hypothalamic astrocytes. By ARC single-cell sequencing, we observed that a HFHS diet dictates time- and cell- specific transcriptomic responses, revealing that astrocytes have the most distinct regulatory pattern compared to other cell types. Lastly, we topographically and molecularly characterized astrocytes expressing glial fibrillary acidic protein and/or aldehyde dehydrogenase 1 family member L1 in the ARC, of which the abundance was significantly increased, as well as the alteration in their spatial and molecular profiles, with a HFHS diet. Together, our results provide a detailed multi-omics view on the spatial and temporal changes of astrocytes particularly in the ARC during different time points of adaptation to a high calorie diet.
Issue Date
2022
Journal
Glia 
Organization
Max-Planck-Institut für Experimentelle Medizin 
ISSN
0894-1491
eISSN
1098-1136
Language
English
Sponsor
Marie Sklodowska-Curie Actions https://doi.org/10.13039/100018694

Reference

Citations


Social Media