Ectosomes and exosomes modulate neuronal spontaneous activity
2022 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Details
- Authors
- Brás, Inês C.; Khani, Mohammad H. ; Riedel, Dietmar ; Parfentev, Iwan; Gerhardt, Ellen ; van Riesen, Christoph; Urlaub, Henning ; Gollisch, Tim ; Outeiro, Tiago F.
- Abstract
- Extracellular vesicles (EVs) are important mediators in intercellular communication. However, understanding the biological origin and functional effects of EVs subtypes has been challenging due to the moderate differences in their physical properties and absence of reliable markers. Here, we characterize the proteomes of ectosomes and exosomes using an improved differential ultracentrifugation protocol and quantitative proteomics. Our analyses revealed singular proteomic profiles for ectosomes and exosomes that enabled us to establish specific protein markers that can be used for their biochemical distinction. Cytoskeleton and glycolytic proteins are distinctively present in ectosomes, while endosomal sorting complexes proteins and tetraspanins are enriched in exosomes. Furthermore, annexin-A2 was identified as a specific marker for ectosomes derived from cell media and human cerebrospinal fluid. Expression of EGFP as a cytosolic reporter leads to its incorporation in EVs and enables their imaging with higher resolution. Assessment of neuronal network activity using multi-electrode array recordings demonstrated that spontaneous neuronal activity can be modulated by EVs. Ectosomes and exosomes internalization in neuronal cells disrupted their regular synchronized bursting activity, resulting in overall lower and more disorganized spiking activity. Our findings suggest that EVs cargoes reflect core intracellular processes, and their functional properties might regulate basic biological and pathological processes.
- Issue Date
- 2022
- Journal
- Journal of Proteomics
- Project
- EXC 2067: Multiscale Bioimaging
SFB 1286: Quantitative Synaptologie
SFB 1286 | B08: Definition von Kaskaden molekularer Veränderungen bei Synucleinopathien während der Neurodegeneration - Organization
- Center for Biostructural Imaging of Neurodegeneration ; Abteilung Experimentelle Neurodegeneration ; Universitätsmedizin Göttingen ; Klinik für Augenheilkunde ; Max-Planck-Institut für Biophysikalische Chemie ; Klinik für Neurologie ; Institut für Klinische Chemie ; Max-Planck-Institut für Multidisziplinäre Naturwissenschaften
- Working Group
- RG Gollisch (Sensory Processing in the Retina)
RG Outeiro (Experimental Neurodegeneration)
RG Urlaub (Bioanalytische Massenspektrometrie)
RG Urlaub (Bioanalytik) - External URL
- https://mbexc.uni-goettingen.de/literature/publications/552
https://sfb1286.uni-goettingen.de/literature/publications/172 - ISSN
- 1874-3919
- Language
- English
- Sponsor
- http://dx.doi.org/10.13039/501100001659 Deutsche Forschungsgemeinschaft
http://dx.doi.org/10.13039/501100004939 Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und Molekulare Biowissenschaften
http://dx.doi.org/10.13039/501100007601 Horizon 2020
http://dx.doi.org/10.13039/501100000781 European Research Council