Structural insights into crista junction formation by the Mic60-Mic19 complex

2022 | journal article. A publication with affiliation to the University of Göttingen.

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​Structural insights into crista junction formation by the Mic60-Mic19 complex​
Bock-Bierbaum, T.; Funck, K.; Wollweber, F.; Lisicki, E.; von der Malsburg, K.; von der Malsburg, A. & Laborenz, J. et al.​ (2022) 
Science Advances8(35) art. eabo4946​.​ DOI: 

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Bock-Bierbaum, Tobias; Funck, Kathrin; Wollweber, Florian; Lisicki, Elisa; von der Malsburg, Karina; von der Malsburg, Alexander; Laborenz, Janina; Noel, Jeffrey K.; Hessenberger, Manuel; Jungbluth, Sibylle; Daumke, Oliver 
Mitochondrial cristae membranes are the oxidative phosphorylation sites in cells. Crista junctions (CJs) form the highly curved neck regions of cristae and are thought to function as selective entry gates into the cristae space. Little is known about how CJs are generated and maintained. We show that the central coiled-coil (CC) domain of the mitochondrial contact site and cristae organizing system subunit Mic60 forms an elongated, bow tie–shaped tetrameric assembly. Mic19 promotes Mic60 tetramerization via a conserved interface between the Mic60 mitofilin and Mic19 CHCH (CC-helix-CC-helix) domains. Dimerization of mitofilin domains exposes a crescent-shaped membrane-binding site with convex curvature tailored to interact with the curved CJ neck. Our study suggests that the Mic60-Mic19 subcomplex traverses CJs as a molecular strut, thereby controlling CJ architecture and function.
The Mic60-Mic19 complex assembles into a tetrameric molecular strut that controls crista junction architecture.
Issue Date
Science Advances 
FOR 2848: Architektur und Heterogenität der inneren mitochondrialen Membran auf der Nanoskala 
FOR 2848 | P06: Strukturelle und funktionelle Studien zu OPA1 zur Untersuchung der Mechanismen, die die Form der inneren mitochondrialen Membran bestimmen. 
FOR 2848 | Z01: Elektronenmikroskopie 
Max-Planck-Institut für Biophysikalische Chemie ; Max-Planck-Institut für Multidisziplinäre Naturwissenschaften ; Klinik für Neurologie ; Universitätsmedizin Göttingen 
Working Group
RG Daumke (Structural Biology of Membrane-Associated Processes) 
RG Jakobs (Structure and Dynamics of Mitochondria) 
RG Riedel 
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