The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis

2018 | journal article; research paper

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​The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis​
Lorenzi, I.; Oeljeklaus, S.; Aich, A. ; Ronsör, C.; Callegari, S. ; Dudek, J.   & Warscheid, B. et al.​ (2018) 
Biochimica et Biophysica Acta1865(2) pp. 323​-333​.​ DOI: 

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Lorenzi, Isotta; Oeljeklaus, Silke; Aich, Abhishek ; Ronsör, Christin; Callegari, Sylvie ; Dudek, Jan ; Warscheid, Bettina; Dennerlein, Sven ; Rehling, Peter 
The three mitochondrial-encoded proteins, COX1, COX2, and COX3, form the core of the cytochrome c oxidase. Upon synthesis, COX2 engages with COX20 in the inner mitochondrial membrane, a scaffold protein that recruits metallochaperones for copper delivery to the CuA-Site of COX2. Here we identified the human protein, TMEM177 as a constituent of the COX20 interaction network. Loss or increase in the amount of TMEM177 affects COX20 abundance leading to reduced or increased COX20 levels respectively. TMEM177 associates with newly synthesized COX2 and SCO2 in a COX20-dependent manner. Our data shows that by unbalancing the amount of TMEM177, newly synthesized COX2 accumulates in a COX20-associated state. We conclude that TMEM177 promotes assembly of COX2 at the level of CuA-site formation.
Issue Date
Biochimica et Biophysica Acta 
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P13: Protein Transport über den mitochondrialen Carrier Transportweg 
Working Group
RG Rehling (Mitochondrial Protein Biogenesis) 
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