Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins

2017 | journal article

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​Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins​
Butkevich, A. N. ; Ta, H. ; Ratz, M. ; Stoldt, S. ; Jakobs, S. ; Belov, V. N.   & Hell, S. W. ​ (2017) 
ACS chemical biology13(2) pp. 1​-6​.​ DOI: https://doi.org/10.1021/acschembio.7b00616 

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Authors
Butkevich, Alexey N. ; Ta, Haisen ; Ratz, Michael ; Stoldt, Stefan ; Jakobs, Stefan ; Belov, Vladimir N. ; Hell, Stefan W. 
Abstract
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.
Issue Date
2017
Journal
ACS chemical biology 
eISSN
1554-8937
Language
English

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