Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities

2022-11-10 | conference paper. A publication with affiliation to the University of Göttingen.

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​Imaging of excised cochleae by micro-CT: staining, liquid embedding, and image modalities​
Schaeper, J. J. ; Liberman, M. C. & Salditt, T.​ (2022)
In:Müller, Bert; Wang, Ge​ (Eds.), ​Proceeding of SPIE. (Vol. 12242). ​SPIE Optical Engineering + Applications, Developments in X-Ray Tomography XIV​, San Diego.
Proc. SPIE 12242​ DOI: https://doi.org/10.1117/12.2632792 

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Authors
Schaeper, Jannis Justus ; Liberman, M. Charles; Salditt, Tim
Editors
Müller, Bert; Wang, Ge
Abstract
Assessing the complex three-dimensional (3D) structure of the cochlea is crucial to understand fundamental aspects of signal transduction in the inner ear and is a prerequisite for the development of novel cochlear implants (CIs). Phase-contrast x-ray tomography (XPCT) offers destruction-free 3D imaging with little sample preparation, thus preserving the delicate structure of the cochlea. In this work, we present XPCT data of small-animal and human cochleae obtained at dedicated synchrotron radiation (SR) and inhouse µ-CT setups. For µ-CT we explore heavy metal stains as radiocontrast agents and show optimized phase retrieval. With the brilliant SR we achieve high contrast for unstained whole cochleae at the cellular level.
Issue Date
10-November-2022
Project
EXC 2067: Multiscale Bioimaging 
SFB 1456: Mathematik des Experiments: Die Herausforderung indirekter Messungen in den Naturwissenschaften 
SFB 1456 | Cluster A | A03: Dimensionality reduction and regression in Wasserstein space for quantitative 3D histology 
Organization
Institut für Röntgenphysik 
Working Group
RG Salditt (Structure of Biomolecular Assemblies and X-Ray Physics) 
Conference
SPIE Optical Engineering + Applications, Developments in X-Ray Tomography XIV
ISBN
9781510654686
9781510654693
Conference Place
San Diego
Event start
2022-08-21
Event end
2022-08-26
Language
English
Subject(s)
x-ray imaging

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