A non-targeted metabolomics analysis identifies wound-induced oxylipins in Physcomitrium patens

2023-01-10 | journal article. A publication with affiliation to the University of Göttingen.

Jump to:Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​A non-targeted metabolomics analysis identifies wound-induced oxylipins in Physcomitrium patens​
Resemann, H. C.; Feussner, K.; Hornung, E. & Feussner, I.​ (2023) 
Frontiers in Plant Science13.​ DOI: https://doi.org/10.3389/fpls.2022.1085915 

Documents & Media

Table_4.xlsx301.47 kBUnknownTable_3.xlsx467.94 kBUnknownTable_2.xlsx18.49 kBUnknownTable_1.xlsx379.97 kBUnknownfpls-13-1085915-g004.tif1.08 MBTIFFfpls-13-1085915-g003.tif690.2 kBTIFFfpls-13-1085915-g002.tif638.06 kBTIFFfpls-13-1085915-g001.tif249.12 kBTIFFfpls-13-1085915.pdf3.81 MBAdobe PDFDataSheet_2.pdf68.66 kBAdobe PDFDataSheet_1.pdf61.14 kBAdobe PDF

License

Published Version

Attribution 4.0 CC BY 4.0

Details

Authors
Resemann, Hanno Christoph; Feussner, Kirstin; Hornung, Ellen; Feussner, Ivo
Abstract
Plant oxylipins are a class of lipid-derived signaling molecules being involved in the regulation of various biotic and abiotic stress responses. A major class of oxylipins are the circular derivatives to which 12-oxo-phytodienoic acid (OPDA) and its metabolite jasmonic acid (JA) belong. While OPDA and its shorter chain homologue dinor-OPDA (dnOPDA) seem to be ubiquitously found in land plants ranging from bryophytes to angiosperms, the occurrence of JA and its derivatives is still under discussion. The bryophyte Physcomitrium patens has received increased scientific interest as a non-vascular plant model organism over the last decade. Therefore, we followed the metabolism upon wounding by metabolite fingerprinting with the aim to identify jasmonates as well as novel oxylipins in P. patens. A non-targeted metabolomics approach was used to reconstruct the metabolic pathways for the synthesis of oxylipins, derived from roughanic, linoleic, α-linolenic, and arachidonic acid in wild type, the oxylipin-deficient mutants of Ppaos1 and Ppaos2, the mutants of Ppdes being deficient in all fatty acids harboring a Δ6-double bond and the C20-fatty acid-deficient mutants of Ppelo. Beside of OPDA, iso-OPDA, dnOPDA, and iso-dnOPDA, three additional C18-compounds and a metabolite being isobaric to JA were identified to accumulate after wounding. These findings can now serve as foundation for future research in determining, which compound(s) will serve as native ligand(s) for the oxylipin-receptor COI1 in P. patens.
Issue Date
10-January-2023
Journal
Frontiers in Plant Science 
eISSN
1664-462X
Language
English
Sponsor
Open-Access-Publikationsfonds 2022

Reference

Citations


Social Media