Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice

2016 | journal article. A publication with affiliation to the University of Göttingen.

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​Inducible targeting of CNS astrocytes in Aldh1l1-CreERT2 BAC transgenic mice​
Winchenbach, J.; Düking, T.; Berghoff, S. A.; Stumpf, S. K.; Hülsmann, S. ; Nave, K.-A.   & Saher, G.​ (2016) 
F1000Research5 pp. 2934​.​ DOI: https://doi.org/10.12688/f1000research.10509.1 

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Authors
Winchenbach, Jan; Düking, Tim; Berghoff, Stefan A.; Stumpf, Sina K.; Hülsmann, Swen ; Nave, Klaus-Armin ; Saher, Gesine
Abstract
Background: Studying astrocytes in higher brain functions has been hampered by the lack of genetic tools for the efficient expression of inducible Cre recombinase throughout the CNS, including the neocortex. Methods: Therefore, we generated BAC transgenic mice, in which CreERT2 is expressed under control of the Aldh1l1 regulatory region. Results: When crossbred to Cre reporter mice, adult Aldh1l1-CreERT2 mice show efficient gene targeting in astrocytes. No such Cre-mediated recombination was detectable in CNS neurons, oligodendrocytes, and microglia. As expected, Aldh1l1-CreERT2 expression was evident in several peripheral organs, including liver and kidney. Conclusions: Taken together, Aldh1l1-CreERT2 mice are a useful tool for studying astrocytes in neurovascular coupling, brain metabolism, synaptic plasticity and other aspects of neuron-glia interactions.
Issue Date
2016
Journal
F1000Research 
Organization
Max-Planck-Institut für Experimentelle Medizin ; Klinik für Anästhesiologie ; DFG Forschungszentrum Molekularphysiologie des Gehirns und Exzellenzcluster Mikroskopie im Nanometerbereich 
ISSN
2046-1402
Language
English

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