NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover

2021-01-11 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Bonnin, E. A., Fornasiero, E. F., Lange, F., Turck, C. W. & Rizzoli, S. O. (2021). ​NanoSIMS observations of mouse retinal cells reveal strict metabolic controls on nitrogen turnover. BMC Molecular and Cell Biology22(1), Article 5​. ​doi: https://doi.org/10.1186/s12860-020-00339-1 

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Authors
Bonnin, Elisa A.; Fornasiero, Eugenio F.; Lange, Felix; Turck, Christoph W.; Rizzoli, Silvio O.
Abstract
Abstract Background Most of the cells of the mammalian retina are terminally differentiated, and do not regenerate once fully developed. This implies that these cells have strict controls over their metabolic processes, including protein turnover. We report the use of metabolic labelling procedures and secondary ion mass spectrometry imaging to examine nitrogen turnover in retinal cells, with a focus on the outer nuclear layer, inner nuclear layer, and outer plexiform layer. Results We find that turnover can be observed in all cells imaged using NanoSIMS. However, the rate of turnover is not constant, but varies between different cellular types and cell regions. In the inner and outer nuclear layers, turnover rate is higher in the cytosol than in the nucleus of each cell. Turnover rates are also higher in the outer plexiform layer. An examination of retinal cells from mice that were isotopically labeled very early in embryonic development shows that proteins produced during this period can be found in all cells and cell regions up to 2 months after birth, even in regions of high turnover. Conclusions Our results indicate that turnover in retinal cells is a highly regulated process, with strict metabolic controls. We also observe that turnover is several-fold higher in the synaptic layer than in cell layers. Nevertheless, embryonic proteins can still be found in this layer 2 months after birth, suggesting that stable structures persist within the synapses, which remain to be determined.
Issue Date
11-January-2021
Journal
BMC Molecular and Cell Biology 
Project
EXC 2067: Multiscale Bioimaging 
SFB 1286: Quantitative Synaptologie 
SFB 1286 | A05: Mitochondriale Heterogenität in Synapsen 
Organization
Institut für Neuro- und Sinnesphysiologie 
Working Group
RG Rizzoli (Quantitative Synaptology in Space and Time) 
External URL
https://mbexc.uni-goettingen.de/literature/publications/121
https://sfb1286.uni-goettingen.de/literature/publications/90
eISSN
2661-8850
Language
English
Sponsor
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Georg-August-Universität Göttingen (1018)

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