Integrative taxonomy using the plant core DNA barcodes in Sumatra's Burseraceae

2023-04-07 | journal article. A publication with affiliation to the University of Göttingen.

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​Integrative taxonomy using the plant core DNA barcodes in Sumatra's Burseraceae​
Teklemariam, D. M.; Gailing, O.; Siregar, I. Z.; Amandita, F. Y. & Moura, C. C. M.​ (2023) 
Ecology and Evolution13(4).​ DOI: https://doi.org/10.1002/ece3.9935 

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Authors
Teklemariam, Daniel M.; Gailing, Oliver; Siregar, Iskandar Z.; Amandita, Fitri Yola; Moura, Carina C. M.
Abstract
Abstract The high diversity and limited floral information in tropical forests often pose a challenge for species identification. However, over the past decade, DNA barcoding has been employed in tropical forests, including Sumatran forests, to enhance floristic surveys. This technique facilitates the discrimination of morphologically similar species and addresses the limitations of conventional species identification, which relies on short‐lived reproductive structures. This study aimed to evaluate the efficiency of matK, rbcL, and the combination of both chloroplast markers for species identification in Burseraceae by employing genetic distance and species tree inference. In this study, we collected 197 specimens representing 20 species from five genera of Burseraceae. The highest percentage of specimens' identification (36%) at the species level was obtained using matK + rbcL, followed by matK (31%), and rbcL (7%). The matK dataset presented the highest interspecific divergence with a mean of 0.008. In addition, a lack of barcode gap was observed in both markers, suggesting potential limitations of the core barcodes for distinguishing Sumatran species within Burseraceae. The monophyly test confirmed five species as monophyletic using Bayesian species tree inferences for matK. Overall, our results demonstrate that matK outperforms rbcL in species identification of Burseraceae, whereas their combination did not enhance species delimitation. To improve the molecular species assignments of this family, future studies may consider including more DNA markers in conjuction with matK, and broadening the availability of reference sequences for species that have not yet been included in the databases. The outcomes of molecular species identification vary depending on the taxonomic group under investigation. Implementation of phylogenomics for species delimitation and diagnostic marker development is strongly recommended for tropical biodiversity assessments, especially for poorly studied clades.
Species identification in tropical forests is often difficult due to its high diversity and limited floral information, therefore DNA barcoding has been applied in tropical regions such as Sumatran forests to boost floristic surveys. In this study, we sequenced 197 specimens representing 20 species from five genera of the family Burseraceae using the barcode markers matK and rbcL. Our results showed that matK discriminated among 97% of the sequence pairs and identified a modest number of species from the Sumatran tropical rainforest. This reinforces the suitability of this marker for molecular species surveys of Sumatran Burseraceae species. image
Issue Date
7-April-2023
Journal
Ecology and Evolution 
Organization
Zentrum für Biodiversität und Nachhaltige Landnutzung ; Fakultät für Forstwissenschaften und Waldökologie ; Büsgen-Institut ; Abteilung Forstgenetik und Forstpflanzenzüchtung 
Language
English
Sponsor
Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659
Open-Access-Publikationsfonds 2023

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