Sharper low-power STED nanoscopy by time gating

2011 | journal article; research paper. A publication with affiliation to the University of Göttingen.

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​Sharper low-power STED nanoscopy by time gating​
Vicidomini, G.; Moneron, G. ; Han, K. Y.; Westphal, V. ; Ta, H. ; Reuss, M. & Engelhardt, J. et al.​ (2011) 
Nature Methods, 8(7) pp. 571​-573​.​ DOI: https://doi.org/10.1038/NMETH.1624 

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Authors
Vicidomini, Giuseppe; Moneron, Gael ; Han, Kyu Young; Westphal, Volker ; Ta, Haisen ; Reuss, Matthias; Engelhardt, Johann; Eggeling, Christian ; Hell, Stefan 
Abstract
Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells.
Issue Date
2011
Journal
Nature Methods 
ISSN
1548-7091
Language
English

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