Mutual Effects of Caveolin and Nerve Growth Factor Signaling in Pig Oligodendrocytes

2010 | review. A publication with affiliation to the University of Göttingen.

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​Mutual Effects of Caveolin and Nerve Growth Factor Signaling in Pig Oligodendrocytes​
Schmitz, M.; Kloeppner, S.; Klopfleisch, S.; Möbius, W. ; Schwartz, P. J.; Zerr, I.& Althaus, H. H.​ (2010)
Journal of Neuroscience Research, 88​(3) pp. 572​-588​.​
Wiley-blackwell. DOI: 

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Schmitz, Matthias; Kloeppner, Sabine; Klopfleisch, Steve; Möbius, Wiebke ; Schwartz, Peter J.; Zerr, Inga; Althaus, Hans H.
Signaling of growth factors may depend on the recruitment of their receptors to specialized microdomains. Previous reports on PC12 cells indicated an interaction of raft-organized caveolin and TrkA signaling. Because porcine oligodendrocytes (OLs) respond to nerve growth factor (NGF), we were interested to know whether caveolin also plays a role in oligodendroglial NGF/TrkA signaling. OLs expressed caveolin at the plasma membrane but also intracellularly. This was partially organized in the classically Q-shaped invaginations, which may represent caveolae. We could show that caveolin and TrkA colocalize by using a discontinuous sucrose gradient (Song et al. [1996] J. Biol. Chem. 271:9690-9697), MACS technology, and immunoprecipitation. However, differential extraction of caveolin and TrkA with Triton X-100 at 4 degrees C indicated that caveolin and TrkA are probably not exclusively present in detergent-resistant, caveolin-containing rafts (CCRs). NGF treatment of OLs up-regulated the expression of caveolin-1 (cav-1) and stimulated tyrosine-14 phosphorylation of cav-1. Furthermore, OLs were transfected with cav-1-specific small interfering RNA (siRNA). A knockdown of cav-1 resulted in a reduced activation of downstream components of the NGF signaling cascade, such as p21Ras and mitogen-activated protein kinase (MAPK) after NGF exposure of OLs. Subsequently, increased oligodendroglial process formation via NGF was impaired. The present study indicates that CCRs/caveolin could play a modulating role during oligodendroglial differentiation and regeneration. (C) 2009 Wiley-Liss, Inc.
Issue Date
Journal of Neuroscience Research 
1097-4547; 0360-4012
Hertie Foundation (HHA)



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