The moss Physcomitrella patens contains cyclopentenones but no jasmonates: mutations in allene oxide cyclase lead to reduced fertility and altered sporophyte morphology

2010 | journal article. A publication with affiliation to the University of Göttingen.

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​The moss Physcomitrella patens contains cyclopentenones but no jasmonates: mutations in allene oxide cyclase lead to reduced fertility and altered sporophyte morphology​
Stumpe, M.; Goebel, C.; Faltin, B.; Beike, A. K.; Hause, B.; Himmelsbach, K. & Bode, J. et al.​ (2010) 
New Phytologist188(3) pp. 740​-749​.​ DOI: https://doi.org/10.1111/j.1469-8137.2010.03406.x 

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Authors
Stumpe, Michael; Goebel, Cornelia; Faltin, Bernd; Beike, Anna K.; Hause, Bettina; Himmelsbach, Kiyoshi; Bode, Julia; Kramell, Robert; Wasternack, Claus; Frank, Wolfgang; Reski, Ralf; Feussner, Ivo
Abstract
P>Two cDNAs encoding allene oxide cyclases (PpAOC1, PpAOC2), key enzymes in the formation of jasmonic acid (JA) and its precursor (9S,13S)-12-oxo-phytodienoic acid (cis-(+)-OPDA), were isolated from the moss Physcomitrella patens. Recombinant PpAOC1 and PpAOC2 show substrate specificity against the allene oxide derived from 13-hydroperoxy linolenic acid (13-HPOTE); PpAOC2 also shows substrate specificity against the allene oxide derived from 12-hydroperoxy arachidonic acid (12-HPETE). In protonema and gametophores the occurrence of cis-(+)-OPDA, but neither JA nor the isoleucine conjugate of JA nor that of cis-(+)-OPDA was detected. Targeted knockout mutants for PpAOC1 and for PpAOC2 were generated, while double mutants could not be obtained. The Delta PpAOC1 and Delta PpAOC2 mutants showed reduced fertility, aberrant sporophyte morphology and interrupted sporogenesis.
Issue Date
2010
Status
published
Publisher
Wiley-blackwell Publishing, Inc
Journal
New Phytologist 
ISSN
0028-646X

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