Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells

2011 | book part. A publication with affiliation to the University of Göttingen.

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​Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells​
Jakobs, S. ; Stoldt, S.  & Neumann, D. ​ (2011)
In:​Müller, Susann; Bley, Thomas​ (Eds.), High Resolution Microbial Single Cell Analytics pp. 1​-19. ​Berlin: ​Springer. DOI: https://doi.org/10.1007/10_2010_81 

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Authors
Jakobs, Stefan ; Stoldt, Stefan ; Neumann, Daniel 
Editors
Müller, Susann; Bley, Thomas
Abstract
Heterogeneity in the shapes of individual multicellular organisms is a daily experience. Likewise, even a quick glance through the ocular of a light microscope reveals the morphological heterogeneities in genetically identical cultured cells, whereas heterogeneities on the level of the organelles are much less obvious. This short review focuses on intracellular heterogeneities at the example of the mitochondria and their analysis by fluorescence microscopy. The overall mitochondrial shape as well as mitochondrial dynamics can be studied by classical (fluorescence) light microscopy. However, with an organelle diameter generally close to the resolution limit of light, the heterogeneities within mitochondria cannot be resolved with conventional light microscopy. Therefore, we briefly discuss here the potential of subdiffraction light microscopy (nanoscopy) to study inner-mitochondrial heterogeneities.
Issue Date
2011
Publisher
Springer
Series
Advances in Biochemical Engineering, Biotechnology 
ISBN
978-3-642-16886-4
ISSN
0724-6145; 0724-6145
Language
English

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