Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group
2012 | journal article; research paper. A publication with affiliation to the University of Göttingen.
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Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group
Kolmakov, K. ; Wurm, C. A. ; Sednev, M. V. ; Bossi, M. L. ; Belov, V. N. & Hell, S. (2012)
Photochemical & Photobiological Sciences, 11(3) pp. 522-532. DOI: https://doi.org/10.1039/c1pp05321c
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- Authors
- Kolmakov, Kirill ; Wurm, Christian Andreas ; Sednev, Maksim V. ; Bossi, Mariano L. ; Belov, Vladimir N. ; Hell, Stefan
- Abstract
- Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (lambda < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group.
- Issue Date
- 2012
- Journal
- Photochemical & Photobiological Sciences
- ISSN
- 1474-905X
- eISSN
- 1474-9092
- Language
- English