TIM29 is a subunit of the human carrier translocase required for protein transport

2016 | journal article; research paper

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​TIM29 is a subunit of the human carrier translocase required for protein transport​
Callegari, S. ; Richter, F. ; Chojnacka, K.; Jans, D. C. ; Lorenzi, I.; Pacheu-Grau, D.   & Jakobs, S.  et al.​ (2016) 
FEBS Letters590(23) pp. 4147​-4158​.​ DOI: https://doi.org/10.1002/1873-3468.12450 

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Callegari, Sylvie ; Richter, Frank ; Chojnacka, Katarzyna; Jans, Daniel C. ; Lorenzi, Isotta; Pacheu-Grau, David ; Jakobs, Stefan ; Lenz, Christof ; Urlaub, Henning ; Dudek, Jan ; Chacinska, Agnieszka; Rehling, Peter 
Hydrophobic inner mitochondrial membrane proteins with internal targeting signals, such as the metabolite carriers, use the carrier translocase (TIM22 complex) for transport into the inner membrane. Defects in this transport pathway have been associated with neurodegenerative disorders. While the TIM22 complex is well studied in baker's yeast, very little is known about the mammalian TIM22 complex. Using immunoprecipitation, we purified the human carrier translocase and identified a mitochondrial inner membrane protein TIM29 as a novel component, specific to metazoa. We show that TIM29 is a constituent of the 440 kDa TIM22 complex and interacts with oxidized TIM22. Our analyses demonstrate that TIM29 is required for the structural integrity of the TIM22 complex and for import of substrate proteins by the carrier translocase.
Issue Date
FEBS Letters 
SFB 1190: Transportmaschinen und Kontaktstellen zellulärer Kompartimente 
SFB 1190 | P01: Untersuchung der Unterschiede in der Zusammensetzung, Funktion und Position von individuellen MICOS Komplexen in einzelnen Säugerzellen 
SFB 1190 | P13: Protein Transport über den mitochondrialen Carrier Transportweg 
SFB 1190 | Z02: Massenspektrometrie-basierte Proteomanalyse 
Working Group
RG Jakobs (Structure and Dynamics of Mitochondria) 
RG Rehling (Mitochondrial Protein Biogenesis) 
RG Urlaub (Bioanalytische Massenspektrometrie) 
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