Mechanics of Individual Keratin Bundles in Living Cells

2014-12-02 | journal article; research paper. A publication with affiliation to the University of Göttingen.

Jump to: Cite & Linked | Documents & Media | Details | Version history

Cite this publication

​Mechanics of Individual Keratin Bundles in Living Cells​
Nolting, J.-F.; Möbius, W.   & Köster, S. ​ (2014) 
Biophysical Journal107(11) pp. 2693​-2699​.​ DOI: https://doi.org/10.1016/j.bpj.2014.10.039 

Documents & Media

1-s2.0-S0006349514011230-main.pdf1.27 MBAdobe PDFExtras10.12 MBUnknown

License

Published Version

Attribution-NonCommercial-NoDerivs 3.0 CC BY-NC-ND 3.0

Details

Authors
Nolting, Jens-Friedrich; Möbius, Wiebke ; Köster, Sarah 
Abstract
Along with microtubules and microfilaments, intermediate filaments are a major component of the eukaryotic cytoskeleton and play a key role in cell mechanics. In cells, keratin intermediate filaments form networks of bundles that are sparser in structure and have lower connectivity than, for example, actin networks. Because of this, bending and buckling play an important role in these networks. Buckling events, which occur due to compressive intracellular forces and cross-talk between the keratin network and other cytoskeletal components, are measured here in situ. By applying a mechanical model for the bundled filaments, we can access the mechanical properties of both the keratin bundles themselves and the surrounding cytosol. Bundling is characterized by a coupling parameter that describes the strength of the linkage between the individual filaments within a bundle. Our findings suggest that coupling between the filaments is mostly complete, although it becomes weaker for thicker bundles, with some relative movement allowed.
Issue Date
2-December-2014
Journal
Biophysical Journal 
Organization
Institut für Röntgenphysik 
Working Group
RG Köster (Cellular Biophysics) 
ISSN
0006-3495
eISSN
1542-0086
Language
English
Subject(s)
cytoskeleton; cellular biophysics

Reference

Citations


Social Media